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nrnA [2017-09-16 17:26:36]
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nrnA [2017-09-16 17:26:36]

oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase
locus
BSU29250
pI
4.67
mw
34.93 kDa
protein length
313 aa Sequence Blast
gene length
939 bp Sequence Blast
function
degradation of RNA oligonucleotides
product
oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase
essential
no
ec
3.1.3.7
synonyms
ytqI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.1|Exoribonucleases]

    • Gene

    Coordinates
    2,995,908 → 2,996,849

    Phenotypes of a mutant

  • The mutant requires cysteine [Pubmed|17586819]
  • inactivation of ''[gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]'' reduces sporulation efficiency to 5% that of wild type cells; delayed entry into sporulation, reduced SigmaG activity, and production of small forespores [Pubmed|26735940]

    • The protein

    Catalyzed reaction/ biological activity

  • Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate (according to Swiss-Prot)
  • oligoribonuclease (nanoRNAse), 3',5'-bisphosphate nucleotidase [Pubmed|17586819]
  • hydrolyzes oligo(deoxy)ribonucleotides in a 5'→ 3' direction [Pubmed|28894100,21087930]
  • hydrolyzes nano-RNAs in a 3'→ 5' direction [Pubmed|28894100]

    • Structure

  • [PDB|5J21] [pubmed|28894100]

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]
    description
    [Pubmed|9387221]
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    Biological materials

    Mutant

  • MGNA-A151 (ytqI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/151 NBRP B. subtilis, Japan]
  • GP2155 (''[gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • BKE29250 (Δ[gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]::erm trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU29250 BGSC], upstream reverse: 
    _UP1_CATTTGATACTCCTTTATTG
    , downstream forward: 
    _UP4_GAGTAGAGGGGAGACCCTCT
  • BKK29250 (Δ[gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]::kan trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU29250 BGSC], upstream reverse: 
    _UP1_CATTTGATACTCCTTTATTG
    , downstream forward: 
    _UP4_GAGTAGAGGGGAGACCCTCT

    • References

  • 17586819,9387221,16479537,19429620,19553197,21087930,22102036,26691161,21768284,24013631,26735940,27834680,28894100